4IL7
Crystal structure of A223 C-terminal domain, a structural protein from sulfolobus turreted icosahedral virus (STIV)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-28 |
| Detector | MARMOSAIC 325 mm CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 65.853, 65.853, 43.673 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.340 - 1.400 |
| R-factor | 0.14136 |
| Rwork | 0.140 |
| R-free | 0.16506 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.939 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.420 |
| High resolution limit [Å] | 1.370 | 1.370 |
| Number of reflections | 21677 | |
| <I/σ(I)> | 23 | |
| Completeness [%] | 93.0 | 89.2 |
| Redundancy | 5.9 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 298 | 10-15%v/v 2-Propanol, 5-10%w/v Polyethylene glycol 4,000, 0.1M Sodium citrate tribasic dihydrate 5.6 , VAPOR DIFFUSION, HANGING DROP, temperature 298K |
