4IKV
Crystal structure of peptide transporter POT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-22 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.761, 94.917, 57.253 |
| Unit cell angles | 90.00, 111.10, 90.00 |
Refinement procedure
| Resolution | 32.347 - 1.900 |
| R-factor | 0.1825 |
| Rwork | 0.181 |
| R-free | 0.21570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SeM-substituted protein |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.053 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 5.160 | 1.900 |
| Rmerge | 0.053 | 0.033 | 0.201 |
| Number of reflections | 35439 | ||
| <I/σ(I)> | 15.6 | ||
| Completeness [%] | 88.2 | 98.8 | 72.2 |
| Redundancy | 2.8 | 4 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 6.5 | 293 | 40% PEG 400, 100 mM ADA-NaOH, 160 mM Li2SO4, 4 mM SrCl2, pH 6.5, lipidic cubic phase, temperature 293K |
