4IKB
Crystal structure of SNX11 PX domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.480, 38.740, 73.460 |
| Unit cell angles | 90.00, 101.13, 90.00 |
Refinement procedure
| Resolution | 34.120 - 1.780 |
| R-factor | 0.1713 |
| Rwork | 0.169 |
| R-free | 0.22130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.732 |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | BALBES |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 72.069 | 34.116 | 1.880 |
| High resolution limit [Å] | 1.776 | 5.630 | 1.780 |
| Rmerge | 0.059 | 0.129 | |
| Rmeas | 0.066 | 0.144 | |
| Rpim | 0.031 | 0.063 | |
| Total number of observations | 4173 | 21005 | |
| Number of reflections | 27836 | ||
| <I/σ(I)> | 17.6 | 23 | 9.8 |
| Completeness [%] | 97.4 | 94.2 | 96.4 |
| Redundancy | 5.2 | 4.6 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | 0.2M ammonium sulfate, 0.1M Sodium cacodylate trihydrate pH 6.5, 30% PEG 8000, vapor diffusion, temperature 293K |
