4IK1

High resolution structure of GCaMPJ at pH 8.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9794
Spacegroup name	P 41 21 2
Unit cell lengths	119.939, 119.939, 98.616
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.928 - 2.000
R-factor	0.1749
Rwork	0.173
R-free	0.20470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.020
RMSD bond angle	1.718
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.930	1.990
High resolution limit [Å]	1.950	1.950
Number of reflections	49938
Completeness [%]	94.4	82

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	289	Tris pH 8.5, (NH4)2SO4, 25% PEG 3350, 0.5% DDAO, VAPOR DIFFUSION, HANGING DROP, temperature 289K