4IJM
Crystal structure of circadian clock protein KaiC A422V mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2009-12-09 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97586 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 131.362, 134.468, 203.936 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.958 - 3.352 |
R-factor | 0.2192 |
Rwork | 0.217 |
R-free | 0.26070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3dvl |
RMSD bond length | 0.007 |
RMSD bond angle | 0.710 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 3.410 |
High resolution limit [Å] | 3.350 | 9.010 | 3.350 |
Rmerge | 0.056 | 0.028 | 0.649 |
Number of reflections | 52376 | ||
<I/σ(I)> | 31.1 | ||
Completeness [%] | 100.0 | 99.6 | 100 |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 5 | 292 | 0.1 M sodium acetate, 1 M sodium formate, pH 5, EVAPORATION, temperature 292K |