4IJ7

Crystal structure of Odorant Binding Protein 48 from Anopheles gambiae (AgamOBP48) with PEG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X12
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X12
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-11
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.07
Spacegroup name	P 21 21 2
Unit cell lengths	80.650, 89.110, 43.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.218 - 2.250
R-factor	0.2008
Rwork	0.198
R-free	0.24550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3pm2
RMSD bond length	0.011
RMSD bond angle	1.153
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.220	2.370
High resolution limit [Å]	2.250	2.250
Rmerge	0.088	0.530
Number of reflections	15437
<I/σ(I)>	18.6	4.4
Completeness [%]	100.0	100
Redundancy	8	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.1 M Tris-HCl, 25% w/v PEG3000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K