4IIW

2.6 Angstrom Crystal Structure of Putative yceG-like Protein lmo1499 from Listeria monocytogenes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-10
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97856
Spacegroup name	I 4
Unit cell lengths	126.899, 126.899, 115.584
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.910 - 2.600
R-factor	0.17317
Rwork	0.171
R-free	0.21500
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.304
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.640
High resolution limit [Å]	2.600	2.600
Rmerge	0.063	0.567
Number of reflections	55066
<I/σ(I)>	26.1	2.4
Completeness [%]	99.6	100
Redundancy	3.9	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	295	Protein: 7.1mg/mL, 0.25M Sodium cloride, 0.01M Tris-HCl pH 8.3, Screen: Trap96 (E8), 0.2M Ammonium sulfate, 0.1M Cacodyl. pH 6.5, 30% (w/v) PEG 8000, VAPOR DIFFUSION, SITTING DROP, temperature 295K