4IIK
Legionella pneumophila effector
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-02-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.250, 71.520, 88.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.540 - 1.600 |
| R-factor | 0.1235 |
| Rwork | 0.121 |
| R-free | 0.16770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.062 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 55.540 | 1.700 | |
| High resolution limit [Å] | 1.600 | 4.690 | 1.600 |
| Rmerge | 0.055 | 0.023 | 0.575 |
| Rmeas | 0.027 | 0.671 | |
| Number of reflections | 50486 | 3811 | 14967 |
| <I/σ(I)> | 14.2 | 45.75 | 2.14 |
| Completeness [%] | 99.2 | 98.4 | 96.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 291 | 1.6M NaCl, 0.1M Na Acetate, pH 4.8, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
