4IIJ

Crystal structure of tubulin-stathmin-TTL-apo complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-01-26
Detector	PSI PILATUS 2M
Wavelength(s)	0.999870
Spacegroup name	P 21 21 21
Unit cell lengths	103.530, 155.910, 181.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.275 - 2.600
R-factor	0.1993
Rwork	0.197
R-free	0.23530
Structure solution method	Difference Fourier
Starting model (for MR)	4i4t
RMSD bond length	0.004
RMSD bond angle	0.805
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.7.3_928)
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.300	2.670
High resolution limit [Å]	2.600	2.600
Number of reflections	90604
<I/σ(I)>	23.5	1.07
Completeness [%]	100.0	99.9
Redundancy	26.7	25.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.7	293	4-10% PEG 4000, 4-6% glycerol, 30 mM magnesium chloride, 30 mM calcium chloride, 100 mM MES/Imidazole , pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K