4IIA

Low resolution crystal structure of the NTF2-like domain of human G3BP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-2
Synchrotron site	MAX II
Beamline	I911-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-27
Detector	MAR CCD 165 mm
Wavelength(s)	1.04
Spacegroup name	P 63 2 2
Unit cell lengths	89.397, 89.397, 70.136
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.006 - 3.300
R-factor	0.3111
Rwork	0.302
R-free	0.35950
Structure solution method	SIRAS and MR
RMSD bond length	0.006
RMSD bond angle	0.551
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AutoSol
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.350	3.390
High resolution limit [Å]	3.300	3.300
Rmerge	0.085
Number of reflections	2719
<I/σ(I)>	15.7	1.5
Completeness [%]	98.7	99.1
Redundancy	10	10.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	1.6 M diammonium phosphate, 0.1 M MOPS, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K