4IFS
Crystal structure of the hSSRP1 Middle domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-21 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97954 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 89.187, 89.187, 82.589 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.930 |
| R-factor | 0.20008 |
| Rwork | 0.199 |
| R-free | 0.22053 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gcl |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.116 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.240 | 1.960 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.087 | 0.496 |
| Number of reflections | 29080 | |
| <I/σ(I)> | 26.618 | 4.857 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.7 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 287 | 15% PEG 6000, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 287.0K |
