4IFQ

Crystal structure of Saccharomyces cerevisiae NUP192, residues 2 to 960 [ScNup192(2-960)]

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-03-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 43 21 2
Unit cell lengths	134.600, 134.600, 234.794
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.760 - 3.250
R-factor	0.1905
Rwork	0.188
R-free	0.24310
Structure solution method	SAD
Starting model (for MR)	Built using AutoBuild (Phenix) and Buccaneer (CCP4)
RMSD bond length	0.014
RMSD bond angle	1.755
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	REFMAC (5.7.0025)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.290
High resolution limit [Å]	3.254	3.250
Number of reflections	64698
<I/σ(I)>	17.5	2.6
Completeness [%]	100.0	100
Redundancy	8.4	8.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	Protein (20 mM Hepes, pH 8.0, 500 mM NaCl, 10% glycerol, 5mM DTT; Reservoir (10% PEG3350, 100mM pottasium iodide); Cryoprotection (30% PEG400 and 25% saturated ammonium sulfate), Vapor Diffusion, Sitting Drop, temperature 298K