4IFF
Structural organization of FtsB, a transmembrane protein of the bacterial divisome
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9701 |
| Spacegroup name | P 61 |
| Unit cell lengths | 87.577, 87.577, 185.110 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.22122 |
| Rwork | 0.219 |
| R-free | 0.25583 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.029 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.390 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.656 | |
| Number of reflections | 35452 | |
| <I/σ(I)> | 4.1 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.1 | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | 2.1 M ammonium sulfate, 0.6 M malonate, 5% glycerol, 100 mM Bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290 K |
