4IF5

Structure of human Mec17

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-04-04
Detector	PSI PILATUS 6M
Wavelength(s)	0.97940
Spacegroup name	P 21 21 2
Unit cell lengths	41.747, 122.297, 37.541
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.700
R-factor	0.1816
Rwork	0.178
R-free	0.21370
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.107
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX (1.7_650)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.700
Number of reflections	21804

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	100 mM Bis-Tris, 200 mM NaCl, 24 % PEG 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K