4IEJ

Crystal structure of a DNA methyltransferase 1 associated protein 1 (DMAP1) from Homo sapiens at 1.45 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-05-31
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 61 2 2
Unit cell lengths	37.353, 37.353, 229.960
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.327 - 1.450
R-factor	0.1984
Rwork	0.197
R-free	0.22590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hm5
RMSD bond length	0.010
RMSD bond angle	0.920
Data reduction software	XDS
Data scaling software	XSCALE (March 15, 2012)
Phasing software	MOLREP
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.327	38.327	1.500
High resolution limit [Å]	1.450	3.120	1.450
Rmerge	0.050	0.035	0.984
Number of reflections	18199	2094	1696
<I/σ(I)>	18.99	46	2.3
Completeness [%]	99.7	99.2	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.20M calcium chloride 20.00% polyethylene glycol 3350, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K