4IEA

14-3-3 isoform sigma in complex with a phosphorylated C-RAF peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-09-02
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	82.100, 111.500, 62.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.500 - 1.700
R-factor	0.1661
Rwork	0.164
R-free	0.20800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ywt
RMSD bond length	0.026
RMSD bond angle	2.182
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.500		1.800
High resolution limit [Å]	1.700	10.000	1.700
Rmerge	0.065	0.023	0.451
Number of reflections	31028	138	4937
<I/σ(I)>	25.44	72.51	6.24
Completeness [%]	97.3	71.9	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	277	95 mM Na-HEPES pH 7.4, 25.6 % PEG 400, 190 mM CaCl2, 5 % Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 277K