4IDU
crystal structure of Schmallenberg virus nucleoprotein
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 273 |
| Detector technology | CCD |
| Collection date | 2012-07-13 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.9218 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.320, 86.440, 77.400 |
| Unit cell angles | 90.00, 101.26, 90.00 |
Refinement procedure
| Resolution | 29.710 - 3.080 |
| R-factor | 0.32283 |
| Rwork | 0.321 |
| R-free | 0.36425 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.531 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.920 | 3.160 |
| High resolution limit [Å] | 3.060 | 3.060 |
| Rmerge | 0.093 | 0.773 |
| Number of reflections | 18304 | |
| <I/σ(I)> | 2 | 2.6 |
| Completeness [%] | 99.4 | 93.8 |
| Redundancy | 7.5 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 297 | 0.2M sodium formate, 20% PEG3350, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
