4IDL
Low melting temperature Anti-Cholera Toxin Llama VHH domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-16 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 56.444, 51.863, 46.481 |
| Unit cell angles | 90.00, 107.81, 90.00 |
Refinement procedure
| Resolution | 26.870 - 2.090 |
| R-factor | 0.20668 |
| Rwork | 0.206 |
| R-free | 0.22180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cfi |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.092 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.870 | 2.160 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.065 | 0.185 |
| Number of reflections | 7586 | |
| <I/σ(I)> | 20.8 | 5.92 |
| Completeness [%] | 98.7 | 87 |
| Redundancy | 3.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | Hampton Research Index 8 (0.1 M Sodium Acetate Trihydrate pH 4.5, 3.0 M NaCl), VAPOR DIFFUSION, SITTING DROP |
