4ICZ

HER2 1221/1222 phosphorylation regulated by PTPN9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Detector technology	CCD
Collection date	2012-05-06
Detector	RAYONIX MX-225
Wavelength(s)	0.98
Spacegroup name	P 21 21 2
Unit cell lengths	73.249, 85.705, 49.781
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.173 - 1.900
R-factor	0.2055
Rwork	0.203
R-free	0.24870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.045
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX ((phenix.refine: 1.6.2_432))

Data quality characteristics

	Overall
Low resolution limit [Å]	80.000
High resolution limit [Å]	1.900
Number of reflections	25510
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	293	20% PEG-4000, 0.2 M potassium thiocyanate, 10% ethylene glycol, 0.1 M Bis-Tris propane pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6.4	293	20% PEG-4000, 0.2 M potassium thiocyanate, 10% ethylene glycol, 0.1 M Bis-Tris propane pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K