4ICW
N-terminal C2 domain of human CEP120
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-04-20 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97260 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.320, 48.880, 66.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| R-factor | 0.21105 |
| Rwork | 0.210 |
| R-free | 0.23207 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.267 |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.029 | 0.522 |
| Number of reflections | 6902 | |
| <I/σ(I)> | 25.8 | 3.06 |
| Completeness [%] | 98.2 | 98.6 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 292 | 25% (v/w) PEG 3000 , pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
