4ICV

Ubiquitin-like domain of human tubulin folding cofactor E - crystal form B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-02
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.8726
Spacegroup name	I 2 2 2
Unit cell lengths	42.270, 65.830, 73.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.450
R-factor	0.16318
Rwork	0.162
R-free	0.18882
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.324
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	SHELXS
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.490
High resolution limit [Å]	1.450	1.450
Rmerge	0.067	0.508
<I/σ(I)>	19.4	3.1
Completeness [%]	99.5	100
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	292	100 mM Bis Tris pH 6.5 and 43 % (v/v) PEG 400 and 10 mM praseodymium (III) acetate, VAPOR DIFFUSION, HANGING DROP, temperature 292K