4ICA

Crystal structure of a C-terminal truncated form of the matrix subunit (p15) of Feline Immunodeficiency Virus (FIV)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-10-06
Detector	PSI PILATUS 6M
Wavelength(s)	0.97239
Spacegroup name	P 1 21 1
Unit cell lengths	28.480, 71.300, 57.060
Unit cell angles	90.00, 99.63, 90.00

Refinement procedure

Resolution	19.676 - 2.700
R-factor	0.2151
Rwork	0.212
R-free	0.27870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	full-length FIV p15
RMSD bond length	0.002
RMSD bond angle	0.417
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MrBUMP
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.770
High resolution limit [Å]	2.700	2.700
Number of reflections	6139
<I/σ(I)>		4.8
Completeness [%]	98.1	97.8
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	292	0.1 M Tris-HCl, 25% (W/v) polyethylene glycol 6000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 292K