4IC9

Crystal structure of the full-length matrix subunit (p15) of the Feline Immunodeficiency Virus (FIV) Gag polyprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-02-23
Detector	PSI PILATUS 6M
Wavelength(s)	0.98011
Spacegroup name	P 21 21 2
Unit cell lengths	53.010, 71.200, 28.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.310 - 2.000
R-factor	0.1937
Rwork	0.191
R-free	0.25490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ecw SIV matrix protein
RMSD bond length	0.007
RMSD bond angle	1.005
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MrBUMP
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.050
High resolution limit [Å]	2.000	2.000
Number of reflections	7647
<I/σ(I)>		4.78
Completeness [%]	99.8	99.8
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	292	0.1 M sodium acetate, 25% (w/v) polyethylene glycol 3,000, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 292K