4IC6
Crystal structure of Deg8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Detector technology | CCD |
| Collection date | 2010-04-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 129.475, 124.175, 93.274 |
| Unit cell angles | 90.00, 132.41, 90.00 |
Refinement procedure
| Resolution | 29.591 - 2.000 |
| R-factor | 0.2 |
| Rwork | 0.198 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3RFD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.122 |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 73478 |
| Completeness [%] | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 0.2M sodium citrate tribasic dihydrate, 16.25% PEG3350, 5% jeffamine M600, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
