4IC4
Crystal structure of Osh3 ORD from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.91997 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.866, 89.200, 96.102 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.690 - 1.500 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.23400 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.077 | 0.347 |
| Number of reflections | 56981 | |
| <I/σ(I)> | 36.1 | 4.4 |
| Completeness [%] | 99.4 | |
| Redundancy | 6.5 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 0.1M MES-NaOH pH 6.0, 25% PEG1500, 0.1M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
