4IBN
Crystal structure of LC9-RNase H1, a type 1 RNase H with the type 2 active-site motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-09 |
| Detector | BRUKER SMART 6500 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.633, 39.913, 50.254 |
| Unit cell angles | 90.00, 102.64, 90.00 |
Refinement procedure
| Resolution | 49.040 - 1.620 |
| R-factor | 0.179 |
| Rwork | 0.177 |
| R-free | 0.21520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ehg |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.022 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.650 |
| High resolution limit [Å] | 1.620 | 4.400 | 1.620 |
| Rmerge | 0.077 | 0.050 | 0.349 |
| Number of reflections | 20537 | ||
| <I/σ(I)> | 43.648 | ||
| Completeness [%] | 99.2 | 94.9 | 96.2 |
| Redundancy | 13.7 | 13.7 | 13.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 100mM Tris-HCl, 200mM MgCl2.6H2O, 30% PEG 4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
