4IAS

HEW Lysozyme by langmuir- blodgett modified vapour diffusion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Wavelength(s)	0.932
Spacegroup name	P 43 21 2
Unit cell lengths	78.730, 78.730, 37.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.670 - 2.600
R-factor	0.2095
Rwork	0.205
R-free	0.30110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.385
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.16)
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	55.671	39.365	2.740
High resolution limit [Å]	2.600	8.220	2.600
Rmerge		0.059	0.640
Total number of observations		1622	7170
Number of reflections	3906
<I/σ(I)>	20.1	10.1	0.9
Completeness [%]	100.0	99.5	100
Redundancy	12.3	10.1	13.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1