4IAN
Crystal Structure of apo Human PRPF4B kinase domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-17 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 73.266, 75.286, 148.003 |
| Unit cell angles | 90.00, 97.21, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.440 |
| Rwork | 0.228 |
| R-free | 0.28780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.039 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.530 |
| High resolution limit [Å] | 2.440 | 5.250 | 2.440 |
| Rmerge | 0.109 | 0.070 | 0.533 |
| Number of reflections | 29966 | ||
| <I/σ(I)> | 7.6 | ||
| Completeness [%] | 99.3 | 99.6 | 93.5 |
| Redundancy | 3.7 | 3.7 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.2M ammonium sulfate, 20-30% PEG 3350 and 0.1M HEPES pH7.0, vapor diffusion, hanging drop, temperature 291K |
