4I99
Crystal structure of the SmcHead bound to the C-winged helix domain of ScpA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-18 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 0.97932 |
| Spacegroup name | P 41 |
| Unit cell lengths | 117.855, 117.855, 94.482 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.460 - 2.300 |
| Rwork | 0.221 |
| R-free | 0.23600 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.090 | 0.363 |
| Number of reflections | 57452 | |
| <I/σ(I)> | 31.84 | 3.54 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 10.4 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 295 | 100mM Bicine, 220mM ammonium phosphate dibasic, 16% polyethylene glycol 3350, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
