4I94
Structure of BSK8 in complex with AMP-PNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2009-11-15 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.999990 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.100, 74.280, 65.520 |
| Unit cell angles | 90.00, 105.73, 90.00 |
Refinement procedure
| Resolution | 39.130 - 1.800 |
| R-factor | 0.176 |
| Rwork | 0.175 |
| R-free | 0.21780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.592 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (1.3.1) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.000 | 1.900 | |
| High resolution limit [Å] | 1.800 | 10.000 | 1.800 |
| Rmerge | 0.053 | 0.026 | 0.262 |
| Rmeas | 0.031 | 0.323 | |
| Number of reflections | 54269 | 335 | 7600 |
| <I/σ(I)> | 17.49 | 38.47 | 4.16 |
| Completeness [%] | 98.7 | 96.5 | 92.8 |
| Redundancy | 3.67 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.7 | 277 | 200mM Tris, 7-12%(w/v) PEG8000, pH 8.7, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
