4I92
Structure of the BSK8 kinase domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-02-05 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54170 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.090, 76.990, 50.570 |
| Unit cell angles | 90.00, 104.86, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.600 |
| R-factor | 0.161 |
| Rwork | 0.160 |
| R-free | 0.18350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.400 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (1.3.1) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.700 | |
| High resolution limit [Å] | 1.600 | 16.000 | 1.600 |
| Rmerge | 0.027 | 0.027 | 0.088 |
| Rmeas | 0.033 | 0.106 | |
| Number of reflections | 33029 | 24 | 5176 |
| <I/σ(I)> | 32.48 | 49.91 | 12.2 |
| Completeness [%] | 96.3 | 61.5 | 91.6 |
| Redundancy | 3.17 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 277 | 100 mM HEPES pH 7.3 and 7-10%(w/v) PEG 8.000, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
