4I8S

Hen Lysozyme protein crystallization via standard hanging drop vapor diffusion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.932
Spacegroup name	P 43 21 2
Unit cell lengths	80.340, 80.340, 37.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.170 - 2.000
R-factor	0.1978
Rwork	0.195
R-free	0.25910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.022
RMSD bond angle	1.677
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.16)
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	56.809
High resolution limit [Å]	2.000
Number of reflections	8682
Completeness [%]	100.0
Redundancy	11.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			VAPOR DIFFUSION, HANGING DROP