4I8Q

Structure of the aminoaldehyde dehydrogenase 1 E260A mutant from Solanum lycopersicum (SlAMADH1-E260A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-02-26
Detector	PSI PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	I 21 21 21
Unit cell lengths	98.040, 118.070, 128.210
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.770 - 2.650
R-factor	0.2123
Rwork	0.209
R-free	0.28210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4i9b
RMSD bond length	0.010
RMSD bond angle	1.230
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.810
High resolution limit [Å]	2.650	2.650
Number of reflections	21922
Completeness [%]	99.2	98.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	PEG 1500, 100mM Imidazol, 10% glycerol, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K