4I8M

Crystal structure of rabbit Ryanodine receptor 1 (residues 1-536) disease mutant V219I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Detector technology	CCD
Collection date	2010-09-09
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97949
Spacegroup name	H 3 2
Unit cell lengths	169.267, 169.267, 305.599
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.940 - 2.800
R-factor	0.23613
Rwork	0.235
R-free	0.25291
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xoa
RMSD bond length	0.005
RMSD bond angle	0.902
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.860	4.430	2.950
High resolution limit [Å]	2.800	3.960	2.800
Number of reflections	41721
Completeness [%]	100.0	100	100
Redundancy	9.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	9		pH 9.0, EVAPORATION