4I8B
Crystal Structure of Thioredoxin from Schistosoma Japonicum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9707 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 90.756, 149.364, 39.881 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.340 - 2.000 |
| R-factor | 0.1891 |
| Rwork | 0.186 |
| R-free | 0.21570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.986 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 37.340 |
| High resolution limit [Å] | 1.996 |
| Rmerge | 0.722 |
| Number of reflections | 18886 |
| <I/σ(I)> | 411.8 |
| Completeness [%] | 100.0 |
| Redundancy | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 30% PEG 3350, 50 mM Bistris pH 6.5 and 10 mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
