4I83

Crystal Structure of (3R)-Hydroxymyristoyl-ACP Dehydratase from Neisseria meningitidis FAM18

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-08-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	72.440, 103.980, 135.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.960 - 2.600
R-factor	0.22523
Rwork	0.224
R-free	0.25589
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1z6b
RMSD bond length	0.011
RMSD bond angle	1.441
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.730	2.610
High resolution limit [Å]	2.500	2.500
Number of reflections	36282
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	290	100mM Sodium Acetate, 7.5% PEG 4000, 10% Glycerol, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 290K