4I7A
GrpN pentameric microcompartment shell protein from Rhodospirillum rubrum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 150.710, 150.710, 150.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.789 - 3.200 |
| R-factor | 0.2682 |
| Rwork | 0.267 |
| R-free | 0.28850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qw7 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.693 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.3.0) |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 106.570 | 106.570 | 3.280 |
| High resolution limit [Å] | 3.200 | 14.310 | 3.200 |
| Rmerge | 0.066 | 0.014 | 0.560 |
| Number of reflections | 9248 | 75 | 700 |
| <I/σ(I)> | 21.02 | 76.43 | 2.99 |
| Completeness [%] | 96.6 | 58.1 | 99.6 |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | 0.1 M sodium chloride, 0.1 M HEPES, pH 7.2, 1.5 M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
