4I78

Crystal structure of a subtype H17 hemagglutinin homologue from A/little yellow-shouldered bat/Guatemala/060/2010 (H17N10)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-09-11
Detector	RAYONIX MX-300
Wavelength(s)	0.97949
Spacegroup name	H 3 2
Unit cell lengths	100.825, 100.825, 760.536
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 3.180
R-factor	0.20533
Rwork	0.203
R-free	0.25774
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ubq
RMSD bond length	0.012
RMSD bond angle	1.656
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.270
High resolution limit [Å]	3.180	3.180
Number of reflections	24997
Completeness [%]	97.6	91.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	295	0.1 M sodium citrate, pH 5.5, 1M LiCl2, and 15% PEG6000, VAPOR DIFFUSION, SITTING DROP, temperature 295K