4I6U

Crystal Structure of a Y37F mutant of the Restriction-Modification Controller Protein C.Esp1396I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-09-15
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.92
Spacegroup name	P 21 21 21
Unit cell lengths	48.610, 81.850, 135.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.790 - 1.970
R-factor	0.1732
Rwork	0.171
R-free	0.21710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.864
Data reduction software	MOSFLM
Data scaling software	Aimless
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall
Low resolution limit [Å]	45.740
High resolution limit [Å]	1.970
Rmerge	0.081
Number of reflections	38881
<I/σ(I)>	22.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	0.1 M sodium sulphate, 0.2 M sodium acetate, 0.1 M Bis Tris propane, 20 % w/v PEG 3350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K