4I6T
Crystal Structure of a T36A mutant of the Restriction-Modification Controller Protein C.Esp1396I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-09-15 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.92 |
Spacegroup name | P 65 |
Unit cell lengths | 65.449, 65.449, 72.290 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.810 - 2.000 |
R-factor | 0.1874 |
Rwork | 0.184 |
R-free | 0.24520 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.019 |
RMSD bond angle | 1.875 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.1.26) |
Phasing software | PHASER (2.5.1) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 56.681 | 44.600 | 2.020 |
High resolution limit [Å] | 1.970 | 9.030 | 1.970 |
Rmerge | 0.437 | 0.026 | 0.026 |
Total number of observations | 1611 | 9506 | |
Number of reflections | 12508 | ||
<I/σ(I)> | 4.2 | 9 | 1.1 |
Completeness [%] | 99.6 | 99.5 | 100 |
Redundancy | 10.4 | 11.3 | 10.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 100 mM Sodium Sulphate, 2.4 M Sodium Malonate, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 277K |