4I6S
Structure of RSL mutant W76A in complex with L-fucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9817 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 103.765, 103.765, 100.834 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.880 - 1.540 |
| R-factor | 0.14925 |
| Rwork | 0.148 |
| R-free | 0.17173 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bt9 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.733 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 51.880 | 51.880 | 1.620 |
| High resolution limit [Å] | 1.540 | 4.860 | 1.540 |
| Rmerge | 0.042 | 0.029 | 0.347 |
| Number of reflections | 41015 | ||
| <I/σ(I)> | 24.5 | 54.9 | 5.1 |
| Completeness [%] | 99.8 | 99.8 | 99.1 |
| Redundancy | 9 | 8.8 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 292 | 0.1M Tris/Bicine, 0.12M Monosaccharides, 30% P550MME/P20K, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 292.0K |
