4I6E
A vertebrate cryptochrome
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 43 |
| Unit cell lengths | 68.935, 68.935, 127.528 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.744 - 2.700 |
| R-factor | 0.2475 |
| Rwork | 0.244 |
| R-free | 0.28710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cvu |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.272 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.8.1_1168) |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.066 | 0.191 |
| Number of reflections | 16379 | |
| <I/σ(I)> | 45.023 | 7.579 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.1 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.1M MES-imadzole, pH6.5, 7.2% PEG8000, 20% Ethylene glycol (v/v), 0.03M NaF,NaBr,NaI, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
