4I65
Crystal Structure of Staphylococcal nuclease mutant V23I/L25V/I72V/I92V
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2011-04-21 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 |
| Unit cell lengths | 47.980, 47.980, 63.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.760 - 1.610 |
| R-factor | 0.19254 |
| Rwork | 0.190 |
| R-free | 0.23911 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.597 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.080 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 14804 | |
| Completeness [%] | 78.3 | 12.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 25mM Sodium Phosphate buffer, 35-60% MPD, Vapor Diffusion, Hanging Drop, Temperature 277K, pH 7.0, VAPOR DIFFUSION, HANGING DROP |
