4I63

Crystal Structure of E-R ClpX Hexamer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	55.219, 201.168, 222.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.056 - 5.709
R-factor	0.3074
Rwork	0.307
R-free	0.31450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hws
RMSD bond length	0.002
RMSD bond angle	0.449
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.5_2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	6.010
High resolution limit [Å]	5.709	12.440	5.709
Rmerge	0.096	0.074	0.352
Number of reflections	7644
<I/σ(I)>	8.9
Completeness [%]	99.4	99.3	96.2
Redundancy	8.5	8.4	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	9	298	2.2 M ammonium sulfate, 0.2 M ammonium bromide, 0.1 M bicine, pH 9.0, VAPOR DIFFUSION, temperature 298K