4I5T

Crystal structure of yeast Ap4A phosphorylase Apa2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97917
Spacegroup name	P 21 21 21
Unit cell lengths	73.760, 74.460, 112.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.844 - 2.300
R-factor	0.2067
Rwork	0.203
R-free	0.27770
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.251
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall
Low resolution limit [Å]	47.490
High resolution limit [Å]	2.300
Rmerge	0.091
Number of reflections	27391
<I/σ(I)>	10.5
Completeness [%]	97.4
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	18% PEG 2K, 0.1M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K