4I5J
PP2A PR70 Holoenzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2006-08-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.10 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 67.610, 67.610, 139.110 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.849 - 2.091 |
| R-factor | 0.197 |
| Rwork | 0.194 |
| R-free | 0.25220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.122 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.180 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Number of reflections | 21335 | |
| <I/σ(I)> | 10.9 | 1.3 |
| Completeness [%] | 94.9 | 61.2 |
| Redundancy | 4.7 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 296 | 50:50 (0.1 M NaH2PO4/0.1 M K2HPO4, 0.1 M MES, 2 M NaCl) 8 mg/ml protein , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
