4I53

Crystal structure of clade C1086 HIV-1 gp120 core in complex with DMJ-II-121

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4C
Synchrotron site	NSLS
Beamline	X4C
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2011-11-15
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.97894
Spacegroup name	C 2 2 21
Unit cell lengths	67.450, 127.670, 192.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.819 - 2.500
R-factor	0.2546
Rwork	0.254
R-free	0.27330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.053
RMSD bond angle	1.308
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	38.580
High resolution limit [Å]	2.500
Number of reflections	29249
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	18-20% (w/v) PEG 1500, 0.1 M CaCl2, 0.1 M imidazole pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K