4I4N
Crystal Structure of the catalytic Cys to Ala mutant of VcHsp31 from Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-11-20 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 103.750, 79.350, 107.650 |
| Unit cell angles | 90.00, 108.67, 90.00 |
Refinement procedure
| Resolution | 29.360 - 1.840 |
| R-factor | 0.243 |
| Rwork | 0.243 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | AUTOMAR |
| Phasing software | AMoRE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Number of reflections | 130793 | |
| Completeness [%] | 91.0 | 91 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG8K, MPD, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
