4I4L

Crystal Structure of Nucleotide-Bound W-W-W ClpX Hexamer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	55.942, 181.903, 201.378
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.476 - 3.698
R-factor	0.3009
Rwork	0.300
R-free	0.32270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hws
RMSD bond length	0.002
RMSD bond angle	0.439
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.5_2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.830
High resolution limit [Å]	3.698	7.960	3.698
Rmerge	0.077	0.037	0.309
Number of reflections	21372
<I/σ(I)>	8
Completeness [%]	93.9	89.8	95.3
Redundancy	3.8	3.6	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.8	298	75 mM sodium acetate, 1.9 M ammonium sulfate, pH 4.8, VAPOR DIFFUSION, temperature 298K