4I47
Crystal structure of the Ribosome inactivating protein complexed with methylated guanine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 77 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-07-21 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | H 3 |
| Unit cell lengths | 131.428, 131.428, 39.808 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.610 - 2.650 |
| R-factor | 0.167 |
| Rwork | 0.165 |
| R-free | 0.19926 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3s9q |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.556 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.760 | 2.740 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.100 | 0.586 |
| Number of reflections | 7364 | |
| <I/σ(I)> | 9.3 | 2 |
| Completeness [%] | 99.3 | 98.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
